| Educational level | Third cycle (PhD students)
Alternatively, Second cycle (master) |
| Last day of application: | March 15, 2018 (proposed) |
| Duration: | Module: 5 days with 2 h lectures, 4 h laboratory works per day |
| Examination: | Take-home assignment, presentation
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| Objectives: | This course is intended to familiarize biochemists with HPLC. Theoretical aspects of each mode of chromatography are discussed in several lectures, providing an understanding of the various modes of chromatography which are now possible using commercially available columns, from reversed phase to affinity. Practical aspects and instrumentation are also covered. The course presents examples and applications of each mode of chromatography in current biochemical practice. |
| Who should attend: | Postgraduate students.
Students should have taken all basic chemistry courses including organic chemistry, physical chemistry, inorganic chemistry, biochemistry and analytical chemistry. |
| Course Outline: | The Origins and Development of Liquid Chromatography. The Theory of HPLC. Instrumentation. High Performance Ion-Exchange Chromatography. High Performance Size Exclusion Chromatography. High Performance Normal Phase Chromatography. High Performance Reversed Phase chromatography. High Performance Reversed Phase Ion-pair Chromatography. High Performance Affinity Chromatography. Practical Aspects. Applications of HPLC: Nucleosides and Nucleotides; Proteins; Peptides and Amino Acids; Lipids; Carbohydrates; Prostaglandins; Leukotrienes and Hydroxyeicosatetraenoic Acids; Steroids; Biogenic Amines; Vitamins; Antibiotics. |
| Course materials: | All course materials will be based on lectures that will be available to the enrolled students via the e-learning platform |
| Certification: | 7 ECTS will be awarded to the students that will pass the examination
Attendees will be awarded a certificate of attendance |
| Note: | This is an open (public) course and may be subject to minimum numbers attending – recommended group size (minimum) 5 students |
